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How to Templates & Excel | Receipt Templates Write a Descriptive Essay about Template. Simple Id a Person. There is something about the Rent Receipt Templates | Receipt Templates, personal essays - sometimes they are referred to as €ścharacter sketches.€ť But it is difficult to Id Card Template. Blue Id Card Id learn how to Templates For Ms & Excel | Receipt Templates write a descriptive essay about a person, because we really do not read them often. We get €śpictures€ť in our heads about Paralegal Format Paralegals characters in Templates Word & Excel, a piece of & Settlement, fiction over Templates For Ms Word & Excel many pages of Cover Mom Hates This Letter, writing; and most non-fiction does not entail character sketches. So, when you are assigned this type of essay, you may be at For Ms Word | Receipt, a loss as to Id Card Template. Id Card how to construct it or even what to Rent Receipt Templates For Ms Word say. We have explored a lot of Ucr Sub-Workgroups Instructions - S&i Framework, information about this kind of paper and Rent Receipt Templates Word & Excel Templates have made a whole article about Id Card Simple Blue Employee Id Card Vector it in Rent Templates, order to help you out. Here are some pretty basic tips and strategies to use as you develop your piece.

You cannot write a character sketch about - Discharge Instructions Framework anyone you do not know intimately. Receipt For Ms & Excel | Receipt. This person can be a member of your family, a close friend, or even a main character in Resume Format Paralegals Options, a novel or movie if you loved it so much you read or saw it many times. You can select a totally fictitious person, of Templates Word & Excel, course, but it is probably wise to - Mark Systems make the person at least a combination of people you know, so that your description €śsounds€ť authentic to a reader. Most fiction writers admit that their major characters are a bit autobiographical or combinations of people they know, because they are just more believable. Also it will help you to Templates | Receipt Templates get more ideas about Template. Employee what to write and you won€™t get lost. If you want you may even have some sort of an Receipt Templates Word & Excel | Receipt interview with the person you are writing about in More Form Template Pdf Cakeboss Create Sample Cake, order to Rent Receipt Templates Word | Receipt know more about & Settlement Systems them. Thus you will present them in a way more realistic and truthful way.

A descriptive essay about a person is a failure, if all you do is Receipt Templates | Receipt Templates describe that individual physically and & Settlement then tell the reader that s/he has three or four personality traits. Physical descriptions should be revealed indirectly, and Receipt Word & Excel those three or four personality traits must be shown be specific words, actions, and - Mark behaviors. Go back and read your favorite short story or novel. Word & Excel Templates. How does the author reveal everything about Resume Statement Example that main character? Chances are s/he does not spend paragraphs of | Receipt, prose describing what that character looks like.

Bits and Template. Id Card Vector pieces are revealed along the way, and often the details are left up to Rent Templates For Ms | Receipt the reader to fil in. How do you know what the Id Card Template. Simple Employee Id Card Template Id, character€™s personality is Receipt Templates Templates like? You get that over time, as that character speaks and takes action throughout the Closing & Settlement, work. Consider these two methods of providing a physical description: Carol has long curly brown hair, brown eyes, and stands about 5€™ 4€ť tall. For Ms. She is slender, and her long legs give a graceful appearance as she walks. Scaffolding Template | Darley Pcm. (Very boring.) Carol has a completely contagious laugh. Rent Templates Word & Excel | Receipt Templates. When she laughs her entire body is involved. Resume Objective Statement. Those long brown curls fly about her face and shoulders, and Rent For Ms Word & Excel all 5€™ 4€ť of her is somehow involved. And when she is Resume Statement Example angry, watch out. Those piercing brown eyes are throwing daggers of light, and those long legs are poised in a true fighting stance, like she is Rent Receipt Templates Word | Receipt Templates ready to Format 019su Mom Hates With Cover go 16 rounds. Same person €“ two different writers.

See the difference? When you don€™t have an entire novel to gradually provide a physical description, you have to Templates For Ms & Excel Templates get creative with the Format This With Cover, way in Rent Word | Receipt Templates, which you do it. There are a lot of tips and pieces of Ucr Sub-Workgroups - S&i Framework, advice from Receipt Templates & Excel Templates, professional writers on Cover Letter 019su Your the web that can help you to Templates & Excel improve your skills in Id Card Template. Simple Blue Vector, writing character€™s description. Also a lot of Rent Receipt Templates For Ms Word & Excel | Receipt Templates, writers like Chuck Palahniuk, Ray Bradbury, Stephen King and others have written whole books about the art of writing so consider reading them too. Describing Personality Traits. Part of learning how to write a descriptive essay about Create Plan a person mastering this art of showing not telling as you reveal his/her personality traits.

Words and behaviors must be used. & Excel | Receipt. Let€™s take a look at Paralegal Options, Carol again. Suppose you have decided that she really has extremes in Rent Receipt Templates Word & Excel, emotions when she is More Contract Form Sample Create Cake happy or sad €“ there doesn€™t seem to be much €śin between€ť with her. So, that is Rent & Excel | Receipt Templates one of the Closing & Settlement Systems, traits that you want to Receipt Templates Word address in your description. You can take what was written above and expand it a bit, still keeping the Id Card Id Card, physical descriptors but now giving specific examples of Rent Receipt For Ms | Receipt, these extremes. You should reveal them in real-life situations. Objective. Incorporate them in a realistic way. Consider this: Carol has extreme emotional responses, both when happy or angry. Rent For Ms & Excel. When she found out Scaffolding Statement Pcm she was accepted to Rent Receipt Templates Word her first choice for college, she threw her head back, long brown curls flying, raised those slender arms toward the Id Card Simple Blue Id Card Vector, sky and immediately broke into dance moves that I had never seen before, as she sang €śDon€™t Stop Believin€™€ť and Receipt Templates & Excel Templates threw those long legs all over More Form Sample the room. And one day, when someone stole a parking space she had been waiting for, I watched her follow that man all the way into the store, shaking her finger and calling him a rude guy and Rent Receipt For Ms Word & Excel | Receipt Templates several other terms I won€™t mention here.

You have now €śproved€ť to Statement Template Pcm the reader that Carol has extreme emotional responses and done so in an engaging way. Rent Receipt For Ms. It€™s important to give such descriptions if you want to keep your writing interesting and Contract Form Sample Create Cake not to be boring. It also helps you to carve your own style and to Templates improve writing skills at Blue Employee Id Card Template Id, all. As you search for Templates, descriptive essay ideas that will make your character €ślive€ť on Format Mom Hates your paper, you can look online for examples of Rent For Ms Templates, character sketch or personal description essays €“ you will find plenty to Cover Letter 019su Your review that will help you see how to Rent Receipt Word Templates formulate your own €śpicture€ť of Toc - Ucr Sub-Workgroups - S&i Framework, your character. It€™s very useful to read other essays if you want to learn how to Templates Word & Excel | Receipt Templates write good papers.

It may also give you plenty of Id Card Employee, new ideas or to Rent Receipt Templates & Excel Templates inspire you to write a descriptive essay. Objective Objective. Generally, in a character sketch essay, you should identify three personality traits that you will present, each in Receipt Templates Templates, a different paragraph. Contract Form Template Pdf Cakeboss. It€™s not advised to describe whole personality as it will become a novel. Instead consider using this scheme. Your introduction will obviously introduce your person, and Templates the traits that you will be covering. & Settlement - Mark. Your conclusion can either wrap those together to Templates Templates explain how complex, or fun, or interesting this individual is. Video: Create A Performance. A conclusion for Rent Receipt For Ms & Excel | Receipt, Carol might be something like this: Living with my sister Carol has been an - Discharge Instructions adventure, to Rent Word & Excel be sure.

And I hope that adventure continues for Video: How To Create A Performance Improvement Plan, years to Templates For Ms Word Templates come, even after we are grown and More Template Pdf Cakeboss Create Cake have our own separate lives.

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myspace resume MySpace Is Public Space When It Comes To Job Search. Entry Level Job Seekers - It's Time To Reconsider the Web. MILWAUKEE, WI--July 26, 2006--In a recent survey,, the #1 Entry Level Job site, found that 47% of college grad job seekers who use social networking sites such as MySpace and Rent Templates Word | Receipt, Facebook have either already changed or plan to Cover Letter Format 019su Your Mom Hates With Cover change the content of their pages as a result of their job search. There is Rent Word & Excel Templates, a growing trend in the number of employers who are Googling candidates to research for additional information, said Brian Krueger, President of This trend has now spilled over to the use of Internet social networking sites, such as MySpace and More Contract Form Template Pdf Cakeboss Cake, Facebook, for screening potential candidates. MySpace has become the most popular social networking site in Word & Excel Templates, the world, with more than 95 million members.

It accounts for Paralegal Format 80% of all visits to online social networking sites, according to Wikipedia. Facebook has the largest number of registered users among college-focused networking sites, also according to Wikipedia. Are social networking sites private or public? It depends on who you ask. It violates the privacy of the applicants, said Shantice Bates, a Mass Communication Major from Virginia Commonwealth University. A person's MySpace or Facebook pages really have nothing to do with their work personality.

Yet the information is still in the public domain. Word & Excel? Is it truly private? The thought of an employer reviewing your information can be a very scary thought to a candidate, especially in the case of a college student or recent grad, commented Krueger. But as long as the information is Closing, posted publicly, it is available to Receipt Templates For Ms Word others and could potentially be a liability to you in your job search. It could keep you from getting the job. Or, worse yet, from even getting the interview in It. Cv Objective Statement, the first placeand usually you wouldn't even know why. So what about the employee who was asked to change the content of her MySpace page as a condition of Rent For Ms Word Templates, her continued employment? Sound a little invasive? Steven Jungman, Division Director of ChaseSource, LP explained that a client of theirs conducted an Internet search for Resume Format Options a current project, using the & Excel, code name of the project as the keyword.

Surprisingly, a project team members MySpace page was found in the search results. Resume Objective Statement? Not only had she listed her role in the project as her current work experience, but also included on Templates For Ms & Excel Templates the site were some very revealing photographs of her in addition to other very personal information. She was required to remove mention of the employer and Example, the project as a condition of her continued employment. My professional recommendation is that individuals realize that the World Wide Web is exactly that, and personal information is a key click away, said Jungman. Common sense should prevail when someone posts something on the web that they would not put on a resume. Krueger recommends, Entry level job seekers who use MySpace and Templates & Excel Templates, Facebook should update their pages to reflect their job search image. If you don't want information seen by employers, don't publish it publicly. Those who have followed the prevailing advice and cleaned up their personal Web pages have been rewarded, sometimes using the Internet to their networking advantage. Consider Catherine Germann, a 2005 Information Technology graduate from Rochester Institute of Id Card Vector, Technology. While she was in the midst of Rent For Ms Word, her job search, she was contacted out of the blue by a recruiter.

He had seen Germann's resume on a job board and promptly Googled her name, finding her personal website, her live journal and noticed that they had a mutual friend. Germann had already cleaned up her personal pages and adjusted the privacy settings to Closing - Mark project a more professional image. Rent | Receipt Templates? Her site helped her in a positive way to Id Card Template. Simple Blue Id Card Template Vector Id build a potential job connection. I always advise my friends to clean up their pages, even if they aren't using their name, said Germann. There are plenty of ways to figure out who someone is even if they are hiding behind a nondescript screen name. Krueger advises college grad job seekers to beware of the image they project. College grad job seekers should avoid anything that might cause a recruiter to say 'Yikes!' when they found you on Receipt Templates Word & Excel the Web, said Krueger. Blue Employee Template Vector Id? Like it or not, MySpace and Facebook are public sites. Rent Templates & Excel? Instead of posting information and photos from that all night party, job seekers can stand out from the crowd by using these sites as an Format Options opportunity to generate a positive first impression. If you wouldn't put it in your resume, don't put it on the Web. Following are the overall survey results:

Have you changed your content at MySpace or Facebook because of your job search? No - 39.9% Yes - 25.9% No, but I plan to - 9.4% I don't use either MySpace or Facebook - 24.8% The survey was conducted nationally using an online poll placed on the home page during June 2006. The results are based on more than 1600 respondents. is the Rent Templates Word Templates, #1 entry level job site on the Internet and is the leader in the field of entry level job search. Brian Krueger is Cover Letter 019su Your Mom Hates With, President and Founder of and author of the best-selling book for entry level job search, College Grad Job Hunter. We have a first-hand experience of how a candidate's Facebook profile ruined his chances of securing a JobBound internship. Right before we interviewed a recent college graduate, we discovered that one of his interests listed on his profile is Receipt Word & Excel | Receipt, Smokin' blunts with the homies and bustin' caps in Paralegal Resume Format Resume Format, whitey and Rent Receipt Templates For Ms & Excel | Receipt Templates, one of his favorite quotes is Ucr Sub-Workgroups - Discharge Instructions Framework, Beware of big butts and a smile.

Our first impression of Receipt Templates Word Templates, our candidate was officially tainted, and he had little hope of regaining a professional image in our eyes. He was not hired. An organization is only as good as its people, so hiring a new employee is a huge responsibility. Template. Simple Blue Employee Vector? We figured he was probably just trying to be funny on Facebook, but his online profile raised doubts about his judgement and professionalism. Word | Receipt? Fortunately, our online search gave us an insight that we discovered before it was too late. It is so important to Letter 019su Your remember what information is accessible through the Internet. As a recruiter we need as much information about our candidates as possible, so we go beyond reference checks and perform Internet searches. It is alarming to Templates For Ms & Excel Templates see what some of the recent college grads have posted on More Contract Form Template Pdf Cakeboss Sample sites such as MySpace and Facebook. I like to take a look and Rent, see what information is Closing & Settlement - Mark, out there about the candidates by performing a Google search. If they have recognitions through sports or the Rent Templates | Receipt, community, that's great information that adds credibility to the candidate. Cover Letter 019su Your Mom Hates Cover Letter? It also helps me create a bond with that candidate because sometimes this information is Receipt Templates Word | Receipt, more telling than a resume and cover letter.

It also shows them that our company cares about who they are. People don't join companies - they join people. The candidate's MySpace page can also give great background information as to who this person really is and Your This Cover Letter, what kind of friends they have. Because MySpace allows for lots of Rent Receipt & Excel | Receipt Templates, customization and personal touches, candidates should realize that recruiters, clients, business associates and colleagues all have access to the Internet and may see how this person acts outside of work. Cover Your Cover? Make sure the Word | Receipt, image you portray on the Internet is how you want people to Paralegal Resume Format see you in all aspects of your life. Receipt Templates For Ms? Keep your page professional and change your voicemail greeting. By no means would information (positive or negative) found on the Internet make or break my decision.

Talent Acquisition Specialist. North Star Resource Group. I personally think it is not the best way to screen a candidate. If someone has not met with a candidate face to face, but you have looked at pictures (potentially) on the Internet that may or may not be flattering, I think you are opening yourself up to scrutiny about hiring practices, and are you discriminating in some way based on what you saw in those pictures (i.e. didn't like how they looked, looked like a wild person, etc). I think most employers should be able to screen candidates out from the questions they ask during the interview process, information from the resume and/or transcripts, and feedback from references or professors. I can see a need, and have researched more senior candidates on Ucr Sub-Workgroups Instructions - S&i Framework the Internet for articles, publications, etc, and Receipt For Ms Word Templates, see a big value in Cover Letter 019su This Letter, this type of screening. If and when you have a similar need from a college student, this would be useful as well. If a recruiter is | Receipt, relying on Simple Blue Employee Template information from Receipt Templates For Ms Word Templates, a personal page to make their ultimate decision, they should be careful. As far as giving advice to students, you could look at the adage of Paralegal Resumes Resume Format Paralegals Format Options, Don't put anything out on the Internet that you would not want to Templates & Excel Templates have to explain to Closing - Mark your mother, father, grandparents, priest, boss, judge, etc, etc. College Relations Manager.

Tyson Foods, Inc. My general theme is that employers should NOT be looking at MySpace and other social networking pages to find candidates, but rather should be pushing content out TO students through these venues. Screening candidates by looking at Rent Receipt Word | Receipt Templates, these pages is not a particularly good way to determine bona fide occupational qualifications. I call this the your-best-candidate-doing-keg-stands-in-a-hula-skirt phenomenon. Resume Options? If as an employer you see this, what do you do with the Receipt Word, information? In many cases, the answer is nothing, because it is not related to their occupational qualifications. Also, I think it is so important that students understand that we are now Googling for candidates posting resumes on line (and with privacy protections) is now a very popular way for recruiters to find candidates. I would never Google a candidate to see what their web presence is but in Letter Format Your Mom Hates This With Cover, looking for Word | Receipt on-line resumes, other pages could come up. Manager of Talent Acquisition.

Osram Sylvania, Inc. I would recommend that job seekers use the Cover 019su This With, parent test. Templates Word Templates? If you are not concerned that your parents will see your online profile, then you should not be concerned that your future employer sees it. Because in many cases they will! Director of Recruiting. Platinum Solutions, Inc.

I do not use MySpace or Facebook as part of the screening process. On the other hand, my son, who is Form, entering college in the fall has shown me the pages of people he knows, the content of which I would give serious question to if I was looking to recruit them. Keith M. Bloomfield. Director of Human Resources. DeSimone Consulting Engineers, PLLC. The scariest thing about Internet social networking sites is that students have no idea that anyone other than friends are watching. There is a false feeling of safety attached to the network you create with these systems. For Ms? While employers may not have access to these programs, newly hired graduates do and they may be more apt to naturally utilize them. Closing & Settlement? As career counseling professionals, we here at Babson will certainly be communicating with our students about the dangers of getting too personal on social networking sites. Word & Excel? I am afraid that until something happens to Closing & Settlement Systems someone they know, many students will dismiss the warnings to beware.

Director, Center for Career Development. I think that information found on social networking sites should not be used by employers when considering candidates for employment. The networks are exactly that, social ones, and For Ms | Receipt Templates, should not be considered as a measure of Format 019su With Cover, professional worth. However, since some employers may use social networking sites to gain information regarding job applicants, I would strongly advise students to remove any potentially incriminating information that would adversely affect their ability to Rent Templates For Ms & Excel get the interview. Employer Relations Coordinator. In my Senior Seminar course, I have added a discussion about Facebook and Paralegal Resumes Resume Resume Format, MySpace to the course content. Receipt Templates & Excel | Receipt Templates? When I first mentioned it in Resume Objective Objective Example, class, I could see by Rent For Ms Word the expression on a couple of student's faces that they probably had information on the web, that they wouldn't want anyone to see. This is just another area that Career Services staff need to be aware of so we can inform our students of the potential harm in a job search. With the Paralegal Resumes Resume Format Resume Options, increasing technology, everyone needs to think through the information that they put on the web. Director of Career Services.

Valley City State University. Consider. if you were a commercial banker and someone came in with a well-documented business plan, I suspect that you would also Google them and their business ideas to be sure you were considering giving your bank's money to the same person who was in Rent Receipt Templates For Ms | Receipt Templates, your office. Template. Employee? In the 21st century, reference checking will include more and more online research. Associate Professor and Internship Coordinator. Mount Union College. I advise students to clean up their digital dirt! One of my students LOST a great job because he slandered his company on We advise students to Google themselves to see what comes up. This is yet another step in the job search process. Rae Ann Ianniello.

Director, Career Development Center. California State University, East Bay. I work with a lot of recruiters coming to Rent Templates & Excel ASU to either interview or attend our career events and job fairs, and Closing & Settlement, I've not heard from any yet who've said they actually use Internet Social Networking sites to obtain info on potential candidates. Of course, they may be doing this and just not telling us. but I think we would be hearing about For Ms it if they were! Associate Director, Career Services. Arizona State University. It is always better to be safe than sorry. If there is the slightest possibility that an Id Card Template. Simple Blue Employee Vector Id employer may get the urge to look at your information on Facebook/MySpace, pictures included, then make sure what's on Rent Templates For Ms Word & Excel Templates there is what you don't mind them viewing. More Template Pdf Cakeboss Create Sample? Believe me there will be some employers that will view it and there is nothing you as a job seeker can do about it. Director, Career Services. Dallas Baptist University.

Most employers I have spoken with cannot afford the time to go sleuthing on Rent Receipt Templates Word & Excel Templates the Internet. But in some cases, especially where one's character is an area of the utmost concern for employers, this may be useful in gaining a window into & Settlement a side of the Rent For Ms & Excel Templates, candidate that would otherwise be unknown. Voyeurism? Perhaps, but it is foolish for a person to allow this to happen. My advice? Be careful with what you publish, and choose friends who respect your privacy and reputation. Someday it may make all the Letter Format 019su Letter, difference. Loyola University New Orleans. With the hype about Receipt For Ms & Excel | Receipt various information resources comes familiarization. Employers who may not have known that external sources of information were so easily accessed may now become fervent researchers.

As a result of Id Card Template. Vector Id, this new found knowledge on behalf of employers, more of Rent Templates Word Templates, our job seekers need to be constantly reviewing the public information that may be obtained by Toc - - Discharge Instructions - S&i perspective employers about them. Receipt Templates Word Templates? Everything from the resume, Facebook, MySpace, Web pages, to - Discharge - S&i the recorded message on the answering machine at home or on Rent Templates For Ms Word the cell phone provides information about them to whoever has access to Resumes Resume Format Format Options that information. Bottom line to all of Rent Templates For Ms & Excel, this is; let the job seeker beware! Murray State University. College students have naively posted incriminating pictures and Letter Your Mom Hates This With Cover Letter, personal information, believing all the while that it only would be viewed by their fellow college students. Their naivety has blinded them to the fact that many of Rent Templates For Ms & Excel | Receipt, these sites are in Resume Objective Objective Statement, the public domain. Although to their credit, who would ever have suspected that employers would turn to such a method to garner information about a candidate?

While some sources don't believe the trend of Rent Receipt Templates | Receipt Templates, recruiters using these sites in their recruiting efforts is wide-spread, it can't be underestimated, and it may also be much more wide spread than many people want to believe. This fall, in Toc - Ucr Sub-Workgroups - Discharge Instructions, our Professional Development Seminar (PDS) Program (a series of Receipt For Ms & Excel | Receipt, 4 courses in career development that are required of every student), we will be teaching our students how to use these sites to Resume Paralegals Resume Format Options their advantage by Rent For Ms Word | Receipt Templates posting positive, informative career information about themselves. If recruiters are going to use these sites for background checks, then why not have them find important items like a student's career goals, their GPA, their internship and Contract Form Template Pdf Cakeboss Sample Cake, work experience, and their specialized skills? Director of Career Services. Employers are within their rights to research people online. The Internet is a public forum, where one's expectation of privacy is almost nonexistent. The trouble with the Information Superhighway is that because we don't readily see the message recipient, we don't have any real concept as to who might be reading our websites. Unless your website is password-protected, personal webpage postings are the cyber equivalent of Templates & Excel | Receipt Templates, grabbing the microphone in the announcer's box at Yankee Stadium and broadcasting your content for all to hear. Are you comfortable with all 50,000+ listening in--regardless of their potential reaction? If the Ucr Sub-Workgroups - Discharge Instructions Framework, answer is anything but an unqualified YES, then it might be a good idea to keep it to yourself for the time being, at least until you have secured a job.

When it comes to Receipt Templates For Ms | Receipt Templates employers using Google and Facebook, I feel they are opening themselves up to lawsuits. Resume Objective It. Cv Statement? In the U.S., students are discouraged from attaching a photo to their resume or revealing personal information that employers can't ask about- marital status, age, race, religion, ethnicity, country of origin, etc; however that information is readily available on Facebook. So employers that use Facebook or even Google may find information that they could never inquire about in the interview. I'm sure there are lawsuits looming on Rent Templates For Ms & Excel the horizon. A wise company should just say no when it comes to using Facebook. Associate Director, Career Center. Johns Hopkins University. Anyone entering the job market should realize that everything posted online to websites, weblogs, or electronic bulletin boards are fair game and open to public scrutiny. For years we have cautioned college graduates to select professional e-mail addresses and to take a serious approach at the messages recorded on voice mail and Paralegal Resumes Resume Format, answering machines. This is simply a new venue to be aware of.

The great news is, these sites provide a real opportunity for self-marketing and promotion. Receipt Templates For Ms | Receipt Templates? Consider this an opportunity for Employment Judo. College grads who take a professional approach at their personal blogs and webspace have an opportunity to post and advertise components of Instructions - S&i, their qualifications typically truncated from a typical resume or application. Be aware that everything you do on Receipt Templates For Ms Word Templates the web may be scrutinized and take advantage of the opportunity! Director, College Career Guidance. Oral Roberts University. I think that employers who are using these sites as part of their screening process are swimming in very muddy ethical waters and Id Card Template. Template, frankly, are going to be hard pressed to Receipt For Ms | Receipt demonstrate a fair and consistent hiring process should they be challenged in a legal action. On the other hand, students need to realize that the Id Card Template. Template, Internet blurs the line between public and Rent Receipt For Ms Word | Receipt Templates, private. Anything that they post, or even that their friends post about them, is Letter Format 019su This Letter, potentially viewable by Rent & Excel | Receipt Templates the entire planet. I've always been a strong advocate of free speech, but it's important to recognize that whether it's ethical or unethical for More Contract Form Template Pdf Cakeboss Sample Cake them to do so, people who you may not want to see it, can and in Rent Receipt Templates & Excel Templates, some cases will.

Assistant Director for Employer Relations, Career Services. My 17 year old son and his friends got into Facebook by pretending to be at - Discharge Instructions - S&i, a University called Superior Quinones with an AOL email address. It would be very easy for an employer to get what ever information they want. University of Tulsa. We think it's acceptable for employers to Google or do an Templates & Excel Templates Internet search of our students and alumni as part of their background check.

If the student has photos or personal items on Cover Format 019su Your This With Cover Letter My Space or Facebook that they don't want employers or their parents to see, then they shouldn't put them up there. We've added this information to our 2006-07 Career Guide, career center website and Rent Receipt Word & Excel, recent workshops. We've also recently heard about a student who checked out Id Card Template. Simple Blue Employee Id Card Template Vector Id, a recruiter's MySpace website and found information and Templates | Receipt, photos that were not in good taste. It. Cv Objective Example? They decided not to Receipt Templates continue the interview process with the recruiter or his/her company. So I guess it works both ways!! Director, Experiential Education and Closing, Career Services. University of Kentucky. Religion teaches the golden rule that philosophically translates into an ethic of reciprocity. Rent Receipt Templates For Ms Word & Excel | Receipt? To prepare for interviews, many students Google the individual who will be interviewing them and they can expect the Template. Id Card, same action from employers. At this point in time, much of the Internet is a free exchange of information. If you don't want something known about you don't post it.

Director, MBA Career Management Center. Johnson School, Cornell University. Put anything you want on Receipt Templates For Ms & Excel | Receipt your Facebook and & Settlement - Mark Systems, MySpace -anything you want your mom, your grandmother and your dream job recruiter to see. Director, Career Development. Castleton State College. Using the Internet to identify and eliminate candidates is a great idea. When you sign up for Facebook or MySpace you are essentially voiding all rights to privacy.

Anyone who puts questionable pictures, info, and/or quotes on their site are foolish to think that what they do on their own time won't affect their business life. B.A. Psychology, 2004. Millersville University of Receipt Templates, Pennsylvania. I think it violates the privacy of applicants, and crosses the Resumes Format Paralegals Resume Options, boundary between work life and personal life. Furthermore, a person's myspace or facebook pages really have nothing to do with their work personality. Employers who screen out applicants based on personal Internet pages are practicing discrimination, and Receipt Templates For Ms & Excel | Receipt Templates, unfairly judging potential applicants. Overall, if employers and hiring managers are going to judge us on Cover 019su Your Mom Hates Letter our MySpace page, then there's no point in perfecting my cover letter and resume.

1976: An applicant should have a perfect resume, and professional appearance. 2006: An applicant should have a perfect resume, a Ph.D and not reveal anything potentially fun or unique about themselves on MySpace. Mass Communication Major, Class of 2006. Virginia Commonwealth University. The only aspects of our lives that are private, thanks to technology, are the aspects that we choose to Rent Templates For Ms | Receipt Templates keep private and take measures to do so. While I agree that it is a bit invasive for an employer to use social networking sites to screen potential employees, those individuals made the choice to post information about themselves on public websites that are accessible to anyone, anywhere. They did it willingly, and should be willing to accept that people who accept their resumes are not prohibited to Resume Objective Statement Example view their MySpace or to Google them. Privacy is becoming more about how much information we reveal rather than how easy it is to Rent Templates Word & Excel | Receipt Templates find it out. There are ways to make your personal social networking page less public, so there ARE options for those of us who simply can't go without having that drunk picture from spring break as our Facebook photo. Marketing, Class of 2005.

University of North Carolina at Charlotte - Belk College of Business. Theoretically, whatever your life is like outside of work should be independent of your life at work, but employers don't have much to go off of, and having controversial, publicly available, information about you just opens areas for doubt. Thus, I advocate playing it safe and not posting anything controversial.

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essay on Rent Word | Receipt hvtn 505 The path forward to an efficacious HIV-1 vaccine will be expedited via the analysis of immune correlates from past and future HIV-1 vaccine efficacy trials. Approaches that aim either to improve upon current findings from a partially efficacious vaccine strategy or approaches toward the Paralegal Resume Resume Format Options generation of broadly neutralizing antibodies by rational immunogen design strategies are likely to be fast-tracked through careful probing of the intersections of immune correlate analyses within and Rent Receipt For Ms & Excel | Receipt, among human vaccine clinical trials. & Settlement. When carefully interrogated for correlates of risk (immune responses and host/virus genetics), each of the efficacy trials (failed or partially efficacious) can provide new information to advance the HIV-1 vaccine development field. In some cases, clues about potentially protective immune responses can be discerned, and Rent Word Templates, of equal importance, potentially detrimental or distracting immune responses can be identified. Immunogen design strategies may benefit from these analyses, such that that immunogens can be paired down to essential components. Id Card Employee Id Card Vector Id. Moreover, insights from these studies on how host and virus genetics play a role in vaccine-induced immunity will enable the advancement of Rent Receipt Templates For Ms Word & Excel Templates, immunogen designs toward globally efficacious vaccines across different populations. Resume Format. The intersection of correlate analyses or studies involving immune responses (innate, cellular, humoral), host genetics (e.g., FcR expression, HLA) and HIV-1 virus sieve analyses (virus sequence analysis of all infections (placebo and vaccine groups) to Templates For Ms Word, determine if there were specific genetic attributes of infecting viruses that were significantly different between the two groups) are areas of the Paralegal Resumes Format Options highest information content for follow-up studies (Figure 1).

In this review, the correlate analyses for HIV-1 vaccine efficacy trials are described, with an Receipt Templates | Receipt Templates, emphasis on & Settlement Systems those studies that examine the intersection of different components and analyses. Due to the uniqueness of each efficacy trial and outcome, each study can provide information regarding the minimum bar to overcome to Receipt Templates For Ms | Receipt, achieve HIV-1 vaccine efficacy. Even when there is no overall vaccine efficacy, the heterogeneity of immune responses among vaccines has enabled follow-up studies to identify associations of immune responses with HIV-1 infection risk in subsets of vaccines. Furthermore, an integral part of understanding immune correlates of protection is to also define patterns of immune responses that associate with non-protective vaccines. One of the major goals for the HIV-1 vaccine field is to define correlates of protection from HIV-1 infection, such that the field has biomarkers that will clearly predict vaccine outcome in - Mark, HIV-1 vaccine efficacy trials.

There have been six HIV-1 vaccine efficacy studies to date [1,2,3,4,5,6] (Table 1), each testing either a different vaccine strategy, or different populations with different risk factors and geographic locations. Receipt Templates For Ms Templates. HIV-1 vaccine efficacy studies have been analyzed for Closing correlates of infection risk; however, a correlate of protection for an HIV-1 vaccine has not yet been identified. Templates Word & Excel. Both the non-efficacious vaccines [7,8,9,10,11] and the one partially efficacious HIV-1 vaccine [12,13,14,15] trial have yielded several correlates of Id Card Template. Simple Blue Id Card Vector, infection risk; although more weight is given to those findings from trials where there was overall vaccine efficacy. In total, three of these studies (VAX004, Step, and RV144) found significant correlations with HIV-1 infection risk/incidence and two of Receipt & Excel | Receipt Templates, these studies (Step and RV144) identified potential sites of immune pressure on the virus (virus sieve). The terminology employed for describing correlates and surrogates for protection from HIV-1 infection have been the focus of ongoing discussion and clarification [16,17,18,19]. It is Toc - - Discharge Instructions - S&i generally agreed upon that for Receipt Templates & Excel any one of the Closing - Mark Systems correlates of infection risk identified in RV144 to be considered a correlate of Rent Templates For Ms & Excel | Receipt Templates, protection that reliably predicts protection from Letter Your Cover Letter, HIV-1 infection, it must be tested and proven in another clinical trial either with the same setting or in a different vaccine setting [18]. Ultimately, it is the latter confirmation of the correlate to HIV-1 vaccine efficacy in a different geographic region, different virus strains, different risk groups, etc . that is the most sought after for the development of a global HIV-1 vaccine. (Efficacy futility determined at first interim analysis after full enrollment ) Vaccine-induced immune responses have been studied to identify immune correlations with infection risk and evidence of virus sieve that can inform the design and evaluation of the next phase of vaccine efficacy trials (shaded boxes indicate reported correlations). Immunizations in two efficacy studies (Phambili/HVTN 503, HVTN 505) were stopped; however, follow-up of Rent For Ms, participants continues. a Vaccine Efficacy (VE) Outcome is noted as “No Efficacy” if there was no overall statistically significant vaccine efficacy; b MSM: Men who have sex with men; c Participants, meeting enrollment criteria, were enrolled from the general population; d TG: Transgender.

3. Cellular Immunity and HIV-1 Vaccine Efficacy Trials. HIV-1 specific CD8 + T cells are associated with control of It. Cv Objective Example, HIV-1 replication as demonstrated in acute HIV-1 infection [26,27,28,29,30] (reviewed in Rent Templates For Ms Word & Excel, McMichael et al. [31]) and in Resumes Format Paralegals Resume Format Options, studies of Receipt For Ms Templates, those rare individuals who can naturally control HIV-1 infection long term [32,33,34,35,36,37]. Moreover, studies in NHP have demonstrated, as proof of concept, that vaccine induced CD8 + T cells can be protective [38,39,40,41]. In two HIV-1 efficacy trials (Step and Phambili) aimed at inducing CD8 + T cell responses (in the absence of HIV-1 envelope specific antibodies), there was no overall association with vaccine efficacy. However, these studies indicated that there were vaccine-elicited CD8 + T cells that could impact virus replication; although not sufficient enough to provide overall decreased risk of infection in the trial (either due to low magnitude/breadth/function of T cell responses and/or pre-existing vector immunity etc. ). HVTN 505, also designed to target the & Settlement - Mark Systems induction of CD8 + T cell responses (as well as HIV-1 envelope specific antibodies), was discontinued and unblinded due to lack of Rent Receipt & Excel, efficacy.

Studies of the magnitude and Closing - Mark, quality of the Rent Receipt & Excel CD8 + T cell response are ongoing. Since there was evidence of increased infections in two of the Ucr Sub-Workgroups - Discharge Instructions - S&i Framework Ad5 vector based vaccine studies, Step and Rent Templates & Excel | Receipt Templates, Phambili [42] but not in 019su Your Cover, HVTN 505 [6], there is a greater emphasis on understanding immune responses to Receipt For Ms Word & Excel | Receipt Templates, vaccine vectors and impact on Paralegal Resumes Resume Format Paralegals Resume Format Options subsequent immunity. Additional studies demonstrated that there may be some cross-reactivity in Receipt Templates For Ms Word Templates, the T cell responses among the different adenovirus serotypes that will have to be tested carefully going forward as vectors are chosen for clinical trials [43]. An alternate interpretation is that perhaps many other types of Closing Systems, HIV-1 vaccines induce some level of CD4 + T cell activation; however, the Rent Receipt Templates For Ms | Receipt Templates key is to have sufficient overall HIV-1 specific immunity to tip the balance toward protection. Despite these setbacks on Cover Letter Format 019su Your Mom Hates Cover understanding how to induce protective CD8 + T cell induced immune responses in human HIV-1 clinical trials, a new paradigm for T cell immunity has emerged from NHP vaccine studies.

Picker and colleagues report on a CMV based vaccine regimen that protects NHPs from infection and Receipt Templates For Ms Word & Excel | Receipt, induces a novel subset of Toc - Ucr Sub-Workgroups Instructions - S&i Framework, CD8 + T cells that can broadly recognize HIV-1 epitopes through class II restriction [38]. The series of | Receipt Templates, papers by Picker and colleagues provide new hypotheses to Format Paralegals Resume, test for Rent Receipt Word | Receipt inducing effective cellular immunity, both in terms of both new immunogen vector design and new subsets of CD8 + T cells to target with vaccine approaches. HVTN 502 (Step) and HVTN 503 (Phambili); Host Genetics and Virus Sieve Analyses. The Step study [3,44] as well as the Phambili study [4] were test of concept studies (double-blind, randomized, placebo-controlled) for induction of HIV-1 specific T cells responses. These trials tested the concept of a replication defective adenovirus serotype 5 (Ad5) vector with clade B HIV-1 genes ( gag , pol nef ) (MRKAd5) to decrease virus load in those who became infected. This vaccine regimen generated antigen specific T cell responses, but overall these responses did not result in vaccine efficacy. An increased rate of Objective Objective, HIV-1 infection was observed in vaccines compared to placebos that was associated with being uncircumcised and having pre-existing Ad5 antibodies (reviewed in Gray et al. [45]). This increased risk was confirmed in follow-up studies that also reported a waning of the increased infection risk over time [46]. In order to understand the lack of efficacy (and enhancement of infection in the vaccine group), follow-up studies were undertaken to understand the immunity that was generated by vaccination. One of the goals of these follow-up studies was to examine potentially protective responses that may be subdominant in the population but may impact either acquisition or disease progression in some of the Rent Receipt Templates For Ms vaccines. Notably, detailed analysis of the “breakthrough” viruses, or viruses that did establish infection despite vaccine-induced immunity, demonstrated that there was selection at specific sites indicating potential vaccine induced T cell specific immune pressure [23].

Notably, the antigen specific CD8 + T cells responses induced by Ad5 vaccination were to HIV-1 epitope hotspots that were different than those generated in natural infection and were also directed to highly variable regions that likely can tolerate escape mutations from vaccine induced immune responses [47]. Host genetics also played a role in Toc - - Discharge - S&i, immunity, since those vaccines with HLA alleles (B*27, B*57, B*58:01), known to be associated with HIV-1 control, had lower viral load [48] and Receipt Templates & Excel, their CD8 + T cells exhibited greater killing in Cover Letter 019su Your Mom Hates This Letter, in vitro assays [49]. Recent analysis of those infected within a year of their last vaccination, identified that total T cell breadth and total magnitude of the immune response after the Rent Templates For Ms Word immunization series and prior to infection significantly correlated with lower mean viral load [50]. A sieve analysis of the Ucr Sub-Workgroups - Discharge Framework Step trial found that there was significant sequence divergence of the infecting viruses compared to the vaccine [23]. However, despite an observed anamnestic T cell response (from vaccine-induced immunity) after infection, the Receipt | Receipt Templates specific T cell responses measured as part of the Simple Id Card Vector Id evaluation of the trial did not correspond with the sieve findings [51]. Moreover, none of the HIV-1 antigen specific T cell responses that were tested were associated with risk of infection in the vaccine recipients [11]. Surprisingly, the Rent Receipt Templates Templates non-HIV specific ELISpot magnitude was a significant direct CoR for HIV-1 infection in the vaccine group. Thus, sieve analyses along with immune correlate analyses can be informative in Id Card Template. Simple Employee Id Card Template, generating new hypotheses about baseline or vaccine-induced responses correlating with infection risk.

4. Humoral Immunity and HIV-1 Vaccine Efficacy Trials. Four of the HIV-1 vaccine efficacy trials induce HIV-1 Env-specific antibody responses by three diverse strategies: recombinant HIV-1 gp120 immunogen alone (VAX003 and VAX004); vector prime with gp120 boost (RV144); and Rent Receipt Templates For Ms Word Templates, DNA prime, vector boost (HVTN 505). Although VAX003 and VAX004 efficacy trials each tested gp120 protein only as an immunogen strategy to induce humoral responses, these trials were distinct in Toc - - Discharge - S&i, that they were conducted in Templates, different risk populations (injection drug users (IDU) vs . men who have sex with men (MSM)) and with different clades of gp120 protein immunogens (B/E vs . B/B). Similarly, both RV144 and HVTN 505 were prime boost strategies, but each tested a different vector (ALVAC vs . rAd5) and in different risk populations (community-based risk in Thailand vs . MSM in the U.S.). Toc - Instructions - S&i. It is unknown whether the same mechanisms of protection against Rent For Ms | Receipt, HIV-1 acquisition will be similar or quite distinct among the different risk populations/transmission routes. Ucr Sub-Workgroups Instructions - S&i. Thus, there are four major differences across these HIV-1 vaccine efficacy trials that need to be considered when comparing studies: (1) differences in the HIV-1 gp120 sequence content; (2) differences in the approach (prime/boost and vector vs . protein); (3) diverse infection risk populations tested by each vaccine; and (4) different geographic locations and clades of HIV-1. 4.1. VAX003 and VAX004: Immune Response and Host Genetics. The VAX003 clinical trial was conducted in high-risk injection drug users, and Templates & Excel | Receipt Templates, the vaccine contained bivalent gp120 (Clades B and Clade E: AIDSVAX B/E) [2].

As part of the primary analysis of vaccine efficacy for VAX003, pre-specified antibody variables (V2 and Blue Id Card Template Vector, V3 binding antibodies to Rent Receipt Templates For Ms Word & Excel Templates, a vaccine strain envelope (A244), gp120 binding antibodies (A244 and MN), CD4 blocking antibodies and neutralizing antibodies to Resume Format, HIV-1 MN) were found not to For Ms & Excel | Receipt, be correlates of risk. Toc - - S&i Framework. The VAX004 clinical trial enrolled high-risk men and women (predominantly men who have sex with men) and the vaccine contained two clade B gp120s (AIDSVAX B/B) [1]. Follow-up studies of vaccine-induced humoral responses in VAX004 reported that higher neutralizing antibody (nAbs) responses to an easy to neutralize virus [7], CD4 blocking antibodies [7], and antibody-dependent cellular virus inhibition (ADCVI) [8] correlated with lower HIV-1 infection risk among those in the vaccine group. Neutralizing antibody responses against more difficult to Receipt For Ms Word | Receipt Templates, neutralize viruses ( i.e ., circulating viruses that are the target of vaccine strategies) were also induced. Toc - Ucr Sub-Workgroups - Discharge Instructions. However, one interpretation is that this level and breadth of response may be below the bar needed for Rent Receipt For Ms & Excel | Receipt Templates efficacy (and, thus, providing some information on where the Id Card Simple Employee Id Card Vector bar sits) [52].

Comparative studies among vaccine efficacy trials can also inform as to what might constitute a potentially protective immune response. Neutralizing antibody responses were higher in & Excel, VAX003 compared to RV144 [53], indicating that higher antibody responses were not by themselves better nor predictive of Cover Your This Cover Letter, a positive outcome. Insights from analyses of host genetics suggest that although there was no overall increased infection in the VAX004 vaccine trial, the Fc? receptor IIIa genotype (VV genotype, encoded by an allele with a valine (V) a position 158) was associated with an Receipt Word & Excel | Receipt Templates, increased rate of HIV-1 infection in Contract Template Pdf Cakeboss Sample Create Sample Cake, low risk vaccines, but not in high risk vaccines [9]. RV144 is the only HIV-1 vaccine efficacy trial in which the Receipt Word | Receipt Templates vaccine showed decreased transmission versus the placebo group, with an estimated vaccine efficacy of 31% [5]. An international collaborating team of scientists completed a case control study reporting that two humoral immune measurements correlated with the risk of HIV-1 infection [12]. IgG antibodies to the V1/V2 region of Resume It. Cv, HIV-1 gp120 correlated with a decreased risk of infection [12,13,14,15], and a plasma HIV-1 envelope-specific IgA score correlated with increased risk of infection in the vaccine arm (decreased vaccine efficacy) [12,54]. Six primary variables were tested in the correlate analyses: plasma Env IgA score, Env IgG avidity, HIV-1 neutralizing antibodies (nAbs), antibody-dependent cellular cytotoxicity (ADCC), V1/V2 Env IgG and Env-specific CD4 + T-cells.

Overall, humoral responses were the predominant immune response in For Ms Word & Excel, this trial, with evidence of vaccine-elicited V2 targeted CD4 + T-cell responses [12,55]. One key insight from the evaluation of the RV144 vaccine trial was that the vaccine immunogen was unique in Letter 019su, its antigenicity ( i.e . , exposure of Rent Receipt Templates For Ms & Excel | Receipt, specific epitopes) compared to other envelope proteins [25,56]. Letter Format This With Cover Letter. Studies of the RV144 protein immunogen demonstrated that certain epitopes were better exposed, as a result of an 11 amino acid N -terminal deletion of the AE.A244 gp120 envelope, thus leading to the induction of Receipt & Excel | Receipt, dominant V1V2 antibody epitope specificities that were well exposed on More Form Sample Cake the vaccine immunogen [56]. In particular, the Receipt Templates & Excel A244 gp120 used in RV144 was antigenic for both linear V2 epitopes bound by strain-specific V2 antibodies, as well as for conformational V1V2-glycan epitopes bound by V1V2 broad neutralizing antibodies (BnAbs) [25,56]. Closing & Settlement - Mark. Thus, 8,000 individuals have been immunized with an antigen expressing a BnAb epitope, yet only Templates Word & Excel, non-glycan-dependent nAbs were induced [12,25,57,58]. Thus, in addition to Resume It. Cv Objective Example, defining correlates of Receipt For Ms & Excel Templates, protection in RV144, a second critical reason to study vaccine responses in RV144 is to understand why BnAbs were not induced by an antigenic immunogen. These findings indicate that careful evaluation of envelope antigenicity ( i.e ., determining what epitopes are exposed for immune recognition) can provide insights into the types of - Discharge Instructions, antibodies that may be elicited by vaccination. 4.2.2.

RV144: Intersection of Antibody Epitope Specificity, Antibody Function and Antibody Form. Further analyses of the six primary variables in the RV144 correlate analyses using interaction models was one way of Rent For Ms Word | Receipt, examining the role of multiple immune measurements in Systems, assessing correlates of HIV-1 infection risk. The secondary analysis as part of the RV144 immune correlate work [12] indicated (through a statistical interaction model) that in the presence of Rent For Ms Word & Excel | Receipt Templates, low vaccine-elicited IgA responses, either ADCC or neutralizing antibody responses correlated with a decreased risk of infection. The ADCC responses induced by RV144 were predominantly to Resume Objective It. Cv Objective Example, the C1 conformational region of gp120 [59,60]; among other epitope specificities ( i.e ., V2, V3) [25]. Thus, one hypothesis from the RV144 correlate study was that C1 region Env-specific IgA could block C1-specific ADCC by binding to the same epitopes on infected cells, but without the functional engagement of NK cells that is needed to mediate ADCC. Receipt Templates & Excel. Indeed, RV144-induced Env IgA was shown to block C1 region-specific IgG-mediated antibody-dependent cellular cytotoxicity by natural killer cells [54]. These studies are an Closing & Settlement Systems, example of how the intersection of Rent Receipt & Excel Templates, multiple approaches can provide insights into vaccine efficacy.

Specifically, this work highlights the Paralegal Format Resume Format need to examine vaccine-induced epitope-specific antibody responses that engage different cellular Fc receptors. 4.2.3. RV144 Secondary Analyses: Supporting the Primary Correlates and For Ms Word | Receipt Templates, Further Hypotheses. Together, the two immune correlates of Paralegal Resumes Paralegals, HIV-1 infection risk in RV144, along with data from the secondary analyses and follow-up studies, indicate that evaluating antibodies that target circulating strains in the population being tested as well as cross-clade epitopes may play a role in understanding vaccine efficacy. Rent Receipt Templates Templates. Circulating Env IgA responses to the C1 region in gp120 of a circulating strain in Thailand (CRF01_AE) was the Cover Letter 019su Your Mom Hates This With Letter strongest statistical correlate of HIV-1 infection risk in Rent For Ms Word | Receipt Templates, RV144.

Moreover, IgG responses to the V2 region of CRF01_AE Env significantly correlated with a decreased risk of infection among the different clades of Objective Objective, V2 responses tested in the peptide microarray assay [12,15]. Further analyses indicate that the breadth, or cross-reactivity to multiple clades, of the V1/V2-specific response induced by RV144 [13,14,25,57,58] may have contributed to the unique nature of the vaccine-induced immune response in RV144. Further studies in other vaccine trials will be able to Rent Templates For Ms Word & Excel Templates, test these specific hypotheses to see if vaccine-induced immunity that can broadly target circulating strains will be critical for vaccine-mediated protection from HIV-1 infection. The RV144 case control study was designed to evaluate potential correlates of infection risk. Notably, multiple analyses support the original primary RV144 immune correlates and follow-up studies involving immune responses and/or host genetics relate to the primary and secondary correlates analysis (Table 2). Letter 019su Your Mom Hates With Cover. These studies indicate that other humoral responses and host genetics may modulate vaccine-induced immunity and impact vaccine efficacy. Virus sieve analyses have complemented humoral immune data demonstrating that different approaches together can provide stronger clues to potential mechanisms for vaccine efficacy. Although there were only six primary immune measurements tested in Receipt For Ms Word Templates, the case control analysis, other measurements as part of the - Discharge - S&i Framework RV144 case control study [12] and as follow-up analyses [13,14,15,54] were significantly associated with HIV-1 infection risk (statistical results with p 0.05) (Table 3). Notably, the Rent Receipt Templates For Ms | Receipt Templates plasma Env IgA correlate of risk was specific to certain HIV-1 Env IgA responses, as not all Env IgA correlated with decreased vaccine efficacy [54] as measured by a binding antibody multiplex assay [61]. 4.2.4.

RV144 Studies: Virus Sieve Analysis and B-Cell Repertoire. For HIV-1 vaccines, immune responses that impact only a proportion of the transmitted viruses can lead to Template. Blue Id Card Template Vector Id, virus sieving, in Rent Receipt Templates For Ms & Excel | Receipt, that specific viruses impervious to vaccine-elicited immunity can establish infection. These virus sieve analyses in Resumes Format Format, concert with probing vaccine-induced immune response can lead to Receipt Templates For Ms Templates, hypotheses of Resumes Resume Format Options, specific immune responses that may prevent the acquisition of some viruses. Rent Word & Excel | Receipt. Moreover, analyses of Objective Example, viruses escaping from Receipt Templates Word | Receipt, vaccine-induced immunity can provide a roadmap for critical targets on infectious virions that need to It. Cv Objective Statement, be targeted by an HIV-1 vaccine to Templates Word Templates, substantially increase vaccine efficacy. Ongoing analyses of the changing complexity of circulating viruses may be critical for understanding the Closing - Mark virus target in Rent For Ms | Receipt, specific populations. Additional independent studies examining breakthrough viruses in RV144 vaccines (sieve analyses) and Template. Blue Employee Id Card Vector Id, the generation of monoclonal antibodies from the B-cells of vaccine recipients have pointed toward a specific site within the V2 region of the HIV-1 envelope. V2 mAbs derived from RV144 vaccines bound to an epitope centered on K169 of the V2 region [25], and a sieve analysis [24] focused on this region found K169 to be a critical site of immune pressure. For Ms | Receipt. Thus, if the infecting strain of HIV-1 matched the Id Card Simple Blue Id Card RV144 vaccine AE.V2 region at K169, the vaccine efficacy was 48%.

Thus, we have proposed that one way to Rent For Ms Templates, enhance the efficacy of follow-up trials to RV144 would be to include additional Envs or V2 peptides with V2 sequences not covered in the original RV144 envelope sequences. Studies of such combinations are currently underway in non-human primates [62]. The functional properties of these V2-specific antibodies include ADCC, neutralization and It. Cv Objective Statement Example, low level virus capture [25,63]; however, it is Templates For Ms Word | Receipt unclear if these findings related to V1/V2 IgG may be mechanistic or non-mechanistic correlates [19]. Thus, the combination of the initial analysis of the V2-directed humoral responses induced by RV144, work identifying that V1V2 (K169) IgG3 correlated with a decreased risk of infection [14], the mAb work that identified the importance of a lysine in position 169 of the V2 loop and - Mark, virology work that resulted in the demonstration of K169 as a site of immune pressure, together, reinforced the hypothesis that the induction of V2-specific antibodies is & Excel | Receipt Templates important to test as a correlate of protection in further HIV-1 vaccine efficacy trials. (1) V2-specific mAbs were isolated and characterized, which involve position 169 in the V2 loop for binding [25]. (2) V1/V2 Env IgG responses are robustly correlated with a decreased risk of Paralegal Resumes Resume Format Options, infection (multiple V1/V2 antigens and two different assays and Templates & Excel | Receipt Templates, laboratories) [13,57]. (3) linear V2 IgG by peptide microarray correlates with a decreased risk of Closing Systems, infection [15]. (4) linear V2 IgG by Receipt Templates & Excel, the binding antibody multiplex assay correlates with a decreased risk of infection in RV144 [64]. (5) IgG3 to Paralegal Resumes Resume Paralegals Resume Options, V1/V2 CaseA2_169K correlates with a decreased risk of Word & Excel | Receipt Templates, infection [14]. The six primary immune measurements evaluated in the RV144 correlate analysis were chosen in part based on each one representing distinct immunological space. Two of these measurements (V1/V2 IgG and Env IgA) significantly correlated with the risk of Simple Employee Id Card Template Vector, HIV-1 infection as reported in Haynes et al [12].

Additionally, the reported results of interaction models indicate potential relationships among the Receipt Templates Word & Excel | Receipt six measurements. Below are the Form Pdf Cakeboss Sample three interactions that were statistically significant. IgG Env avidity. Neutralizing Antibody (NAb) Score. A series of immune measurements, in addition to the preselected primary six variables, were evaluated for correlations with the risk of HIV-1 infection. Templates For Ms Word. Of all the secondary immune variables, eight of these had a p -value of Resume Objective It. Cv Statement Example, 0.05. Three of these are related to Rent Receipt Templates For Ms | Receipt Templates, the direct correlation with plasma Env IgA; two are related to the indirect correlation with Env IgG responses, and three are related to cellular responses ( i.e ., peripheral blood mononuclear cell (PBMC) derived cytokines). A series of immune measurements, in addition to the preselected primary six variables, were evaluated for Resume It. Cv Example correlations with the risk of HIV-1 infection. Of all the Rent For Ms Word | Receipt secondary immune variables, eight of these had a p -value of 0.05. Three of these are related to the direct correlation with plasma Env IgA; two are related to Resumes Paralegals Options, the indirect correlation with Env IgG responses, and Receipt Templates For Ms & Excel, three are related to cellular responses ( i.e ., PBMC derived cytokines).

4.2.5. Multiple Antibody Specificities and Function: Vaccine Efficacy. Due to the natural redundancy of the immune system [18], HIV-1 vaccines may induce multiple potentially protective immune responses. HIV-1 vaccines can induce a broad repertoire of HIV-1 epitope specificities and antibody isotypes/subclasses with a variety of different antiviral functions. & Settlement - Mark. Even in RV144, where V1/V2 Env IgG responses were identified as an Rent Receipt Templates & Excel | Receipt Templates, immune correlate of risk of HIV-1, there was broad heterogeneity among vaccines in their immune responses with a diverse repertoire of antibody forms, specificities and functions [63].

Some of Toc - Ucr Sub-Workgroups, these diverse immune measurements were not tested in the immune correlates analysis, due to inherent assay limitations, sample limitations and the requirement of minimizing test variables in order to maintain statistical power. The interaction models from RV144, as well as several RV144 follow-up studies that examine several antibody specificities and/or function [54] support the Templates For Ms Word & Excel | Receipt hypothesis that multiple antibody specificities with different functions may act in More Form Create, concert. Rent Word | Receipt. Evaluating multiple antibody specificities and antiviral functions, in addition to Id Card Template. Employee Vector Id, identifying single correlates of infection risk, will be central to Receipt Word & Excel | Receipt Templates, identifying mechanistic correlates of protection for HIV-1 toward the goal of identifying an Objective It. Cv Example, efficacious vaccine strategy. 5. Time-Dependent Immune Measurements and Vaccine Efficacy. One important insight from vaccine efficacy studies is the Receipt For Ms & Excel impact of Your, time on vaccine-induced immunity. The time point at which vaccine efficacy is determined in a trial can dramatically influence the resulting level of Receipt For Ms Word | Receipt, vaccine efficacy. RV144 had a 60.5% vaccine efficacy through 12 months after initial vaccination that declined over time [70]. Contract Template Pdf Cakeboss Cake. The reported vaccine efficacy was pre-specified to be reported at 42 months post follow-up and was 31.2% at that time . This higher level of vaccine efficacy at an earlier time point is hypothesized to be due to a higher level or quality of antibody response that decreased over time For example, Env IgG3 responses are induced in Rent Receipt Templates For Ms, RV144, but decline more rapidly than the overall Env IgG response [14]. Current vaccine strategies aim to Ucr Sub-Workgroups - Discharge - S&i, improve the durability of the anti-envelope antibody response through different adjuvant/immunogen combinations. For Ms & Excel | Receipt Templates. Thus, the half-life of Env-specific antibody responses will likely be a critical component for evaluating further HIV-1 vaccine candidates.

Vaccine-induced cell-mediated immunity also depends on Template. Blue Employee Vector time post-vaccination. Cellular immune responses, as well as humoral responses, can wane post-vaccination. Word & Excel | Receipt Templates. A recent study [50] reported that CD8 + T-cell immunity that correlated with decreased virus load post-breakthrough infection was potentially related to time since vaccination. HIV-1 vaccines that can induce an Closing - Mark, orchestrated response comprised of | Receipt, all arms of the immune response (innate, cellular and humoral) may prove to be the most efficacious across different populations, genders and Id Card Simple Blue Employee Template Id, routes of transmission. Thus, it is imperative to advance forward with strategies for both effective T- and B-cell responses that likely will be induced through specific engagement of the innate immune system. New technologies and approaches have allowed the Rent Word | Receipt Templates field to cast a broader net in evaluating vaccine strategies.

The advent of virus sequencing at Id Card Template. Employee Template Vector, the single genome level has allowed for a detailed analysis of the specificities of immune responses that can impact virus replication in both human and Rent Templates & Excel Templates, non-human primates. These virus-specific approaches for that are not sensitive to vaccine-induced immunity compared to More Contract Pdf Cakeboss Sample Sample Cake, those viruses that are sensitive; (2) potential insights into important vulnerable sites (and their structure) on the virion that vaccines should target; and (3) information on whether the described vaccine-induced humoral and Templates Word, cellular immune responses have epitope specificities that match those identified as sieve sites in the virus. New approaches towards understanding systems biology contributes to Letter Mom Hates, a better understanding of the interplay between the vaccine-induced host immune response and virus. Taken together, these technological advances have yielded a robust amount of data that has an unprecedented need for Templates For Ms & Excel Templates innovation in the way the data are analyzed. Analysis of host genetics, viral genetics and immune responses [71,72] have already provided numerous insights into both innate and adaptive responses of virus control and disease progression in HIV-1 infected individuals. & Settlement. Follow-up studies provide evidence of an interplay of Rent Receipt Templates Word & Excel | Receipt Templates, vaccine-induced immunity against the background of Cover Letter Format 019su Your Mom Hates With Cover, host genetics. Understanding the role of host genetics and the functional attributes of vaccine-induced immunity can enable a better understanding of which immune correlates will be broadly applicable in Rent Receipt For Ms Word & Excel Templates, a globally efficacious vaccine.

Similarly, studies are ongoing to understand if immune responses to one vaccine can be predicted by immunity to another vaccine; thus, also allowing deeper insights into intrinsic population effects that may not be distinct to the vaccine strategy being tested. Currently licensed vaccines for other pathogens are based on empirical studies, and Paralegal Resumes Resume Format Paralegals Resume Format Options, many do not have defined mechanistic correlates of protection. The protective efficacy of some of Rent Templates & Excel, these vaccines are associated with antibody responses, with the exception of rotavirus and BCG ((Bacillus Calmette-Guerin) tuberculosis vaccine) [18,73]. Notably, these antibody responses are of a low titer (1:40 titer with vaccines for Resume Objective It. Cv Statement Example smallpox, polio, Japanese encephalitis [74]), and the associated functional properties are not well known [75]. Mucosal IgG has been associated with the protection of several vaccines (e.g., Hib glycoconjugates, meningococcal conjugates, pneumococcal conjugates, polio (Sabin)); whereas mucosal IgA has been associated with the protection of a smaller number of vaccines (influenza intranasal, polio Sabin, rotavirus) (reviewed in [73]). Receipt Templates. For now, a decreased risk of infection by HIV-1 vaccination is associated with antigen-specific serum antibody responses, with some evidence that certain vaccine-induced cellular responses may impact virus replication and that host genetics may modulate vaccine immunity.

Analyses of the correlates of Letter Format Your Mom Hates Cover Letter, HIV-1 infection risk have demonstrated that there are intersections among different analyses (involving immune responses, virus and host genetics), thus providing the areas of highest information content for understanding the potential mechanisms of protective immunity. Identification and rigorous characterization of these areas of overlap are likely to Templates For Ms & Excel, be transformative for the next phase of HIV-1 vaccine design and evaluation. We thank Peter Gilbert, Jerome Kim, Nelson Michael and Julie McElrath for insightful discussion in the preparation of this manuscript. The authors acknowledge funding from the HIV-1 Vaccine Trials Network (HVTN) (5U01 AI46725-05), the Duke University Center for AIDS Research (CFAR) Grant (P30 AI 64518), the Center for HIV/AIDS Vaccine Immunology-Immunogen Discovery (CHAVI-ID), National Institutes of Health/National Institute of Allergy and Infectious Diseases/ Division of AIDS and Bill and Melinda Gates Foundation Grants (1033098, 1032144, 1040758).